Fig 1: Expression of GAL, GALP, GHRL, NPY, NPY2R and GHSR in the hypothalamus.a-c: protein expression of GAL, GALP and GHRL in hypothalamus; d-i: mRNA expression of GAL, GALP, GHRL, NPY, GHSR and NPY2R in hypothalamus; N, normal group; M, model group; EA, electroacupuncture group; SA, sham acupuncture group; GAL, galanin; GALP, galanin-like peptide; NPY, neuropeptide Y; GHRL, ghrelin; GHSR, ghrelin receptor; NPY2R, neuropeptide Y2 receptor; The values are shown as mean ± SEM. N vs M, * P < 0.05, ** P < 0.01; EA vs M, # P < 0.05, # # P < 0.01; SA vs M, ^ P < 0.05, ^ ^ P < 0.01.
Fig 2: Expression of GHRL, NPY, NPY2R and GHSR in the arcuate nucleus (ARC) (a-d). N, normal group; M, model group; EA, electroacupuncture group; SA, sham acupuncture group; NPY, neuropeptide Y; GHRL, ghrelin; NPY2R, neuropeptide Y receptor; GHSR, ghrelin receptor; ARC, arcuate nucleus. The values are shown as mean ± SEM. N vs M, * P < 0.05, ** P < 0.01; EA vs M, # P < 0.05, # # P < 0.01; SA vs M, ^ P < 0.05, ^ ^ P < 0.01.
Fig 3: Changes of body weight and serum levels of metabolic indicators.a: body weight; b: FBG level; c: serum insulin level; d: serum NPY level; e: serum GHRL level; N, normal group; M, model group; EA, electroacupuncture group; SA, sham acupuncture group; FBG, fasting blood glucose; NPY, neuropeptide Y; GHRL, ghrelin; The values are shown as mean ± SEM. N vs M, * P < 0.05, ** P < 0.01; EA vs M, # P < 0.05, # # P < 0.01; SA vs M, ^ P < 0.05, ^ ^ P < 0.01. Eight rats per group were used in each experiment.
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